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Detailed Information
| Description | InVivoPlus anti-mouse PD-1 |
| Item number | 8094110 |
| specification | 5 mg |
| Price | US$ 860.00 |
Description
Product Details
The RMP1-14 monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments. Like the J43 antibody the RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1.Specifications
| Isotype | Rat IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus rat IgG2a isotype control, anti-trinitrophenol |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Syrian Hamster BKH cells transfected with mouse PD-1 cDNA |
| Reported Applications | in vivo blocking of PD-1/PD-L signaling |
| Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Aggregation* | <5% Determined by SEC |
| Purity | >95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| RRID | AB_10949053 |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests* | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
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Product Catalog---SAINING Biotechnology
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Efforts to build a high-end brand of laboratory consumables.
SAINING was established in May 2018, settled in Taizhou (Xianju) Medical Device Industrial Park in February 2019, and established SAINING (Suzhou) Biotechnology Co., Ltd. in March 2020. We are a biological laboratory consumables manufacturer integrating R&D, production and sales.
Since its establishment, the company has developed rapidly and now has a 100,000-level purification workshop of 15,000 square meters, a factory area of 30,000 square meters, a Suzhou technology research and development center, a Suzhou production base and a Taizhou production base. The main products include cell culture, biological detection liquid processing, medical equipment, safety protection, etc., which can be widely used in testing institutions (IVD), biological research, medical treatment, new drug research and development, laboratory scientific research and other fields. The product has performance and high quality. After testing by third-party testing agencies and relevant scientific research units, it has the ability to replace high-quality imported products.
Since its establishment, the company has developed rapidly and now has a 100,000-level purification workshop of 15,000 square meters, a factory area of 30,000 square meters, a Suzhou technology research and development center, a Suzhou production base and a Taizhou production base. The main products include cell culture, biological detection liquid processing, medical equipment, safety protection, etc., which can be widely used in testing institutions (IVD), biological research, medical treatment, new drug research and development, laboratory scientific research and other fields. The product has performance and high quality. After testing by third-party testing agencies and relevant scientific research units, it has the ability to replace high-quality imported products.
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